This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hunt, J. E. Articles by Krilis, S. A. Search for Related Content PubMed PubMed Citation Articles by Hunt, J. E. Articles by Krilis, S. A. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Autoimmune Diseases Social Bookmarking                         What's this?

A Phospholipid-ß2-Glycoprotein I Complex Is an Antigen for Anticardiolipin Antibodies Occurring in Autoimmune Disease But Not with Infection

University of New South Wales, School of Medicine, St George Hospital Kogarah, Sydney, Australia

H. Patrick McNeil

University of New South Wales, School of Medicine, St George Hospital Kogarah, Sydney, Australia

Gary J. Morgan

University of New South Wales, School of Medicine, St George Hospital Kogarah, Sydney, Australia

Regina M. Crameri

University of New South Wales, School of Medicine, St George Hospital Kogarah, Sydney, Australia

Steven A. Krilis

University of New South Wales, School of Medicine, St George Hospital Kogarah, Sydney, Australia

Anticardiolipin antibodies (aCL) purified from patients with autoimmune disease have recently been shown to interact with a phospholipid-binding plasma protein, ß₂-glycoprotein I (ß₂-GPI). The aim of this study was to determine whether aCL purified from patients with infection also interact with ß₂ -GPI.

aCL purified from 23 patients with malaria, infectious mononucleosis, tuberculosis, hepatitis A or syphilis did not require the presence of ß₂-GPI to bind cardiolipin (CL). In contrast, aCL were purified from 11 out of 12 patients with autoimmune disease that bound CL only in the presence of ß₂-GPI. Thrombotic complications appear to be associated with aCL occurring in autoimmune disease but not with aCL associated with infections. We postulate that this increased risk of thrombosis in the autoimmune group may be due to the presence of aCL that bind CL in association with ß₂-GPI, a plasma protein with anticoagulant activity.

Key Words: Anticardiolipin antibodies • ß2-Glycoprotein I • Thrombosis

Lupus, Vol. 1, No. 2, 75-81 (1992)
DOI: 10.1177/096120339200100204


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. M. Calvo-Romero and E. M. Lima-Rodriguez Anticardiolipin Antibodies in Patients with Type 2 Diabetes Mellitus Clin. Med. Res., September 1, 2009; 7(3): 93 - 95. [Abstract] [Full Text] [PDF]

				B. Giannakopoulos, F. Passam, Y. Ioannou, and S. A. Krilis How we diagnose the antiphospholipid syndrome Blood, January 29, 2009; 113(5): 985 - 994. [Abstract] [Full Text] [PDF]

				T. Avcin and E. Silverman Review: Antiphospholipid antibodies in pediatric systemic lupus erythematosus and the antiphospholipid syndrome Lupus, August 1, 2007; 16(8): 627 - 633. [Abstract] [PDF]

				S. M. Alam, M. McAdams, D. Boren, M. Rak, R. M. Scearce, F. Gao, Z. T. Camacho, D. Gewirth, G. Kelsoe, P. Chen, et al. The Role of Antibody Polyspecificity and Lipid Reactivity in Binding of Broadly Neutralizing Anti-HIV-1 Envelope Human Monoclonal Antibodies 2F5 and 4E10 to Glycoprotein 41 Membrane Proximal Envelope Epitopes J. Immunol., April 1, 2007; 178(7): 4424 - 4435. [Abstract] [Full Text] [PDF]

				B. Giannakopoulos, F. Passam, S. Rahgozar, and S. A. Krilis Current concepts on the pathogenesis of the antiphospholipid syndrome Blood, January 15, 2007; 109(2): 422 - 430. [Abstract] [Full Text] [PDF]

				Y Levy, O Almog, A Gorshtein, and Y Shoenfeld The environment and antiphospholipid syndrome Lupus, November 1, 2006; 15(11): 784 - 790. [Abstract] [PDF]

				J. Zhang and K. R. McCrae Annexin A2 mediates endothelial cell activation by antiphospholipid/anti-{beta}2 glycoprotein I antibodies Blood, March 1, 2005; 105(5): 1964 - 1969. [Abstract] [Full Text] [PDF]

				A. Rahman Autoantibodies, lupus and the science of sabotage Rheumatology, November 1, 2004; 43(11): 1326 - 1336. [Abstract] [Full Text] [PDF]

				R Cervera, R A Asherson, M L Acevedo, J A Gomez-Puerta, G Espinosa, G de la Red, V Gil, M Ramos-Casals, M Garcia-Carrasco, M Ingelmo, et al. Antiphospholipid syndrome associated with infections: clinical and microbiological characteristics of 100 patients Ann Rheum Dis, October 1, 2004; 63(10): 1312 - 1317. [Abstract] [Full Text] [PDF]

				S. A. Robertson, C. T. Roberts, E. v. Beijering, K. Pensa, Y. Sheng, T. Shi, and S. A. Krilis Effect of {beta}2-glycoprotein I null mutation on reproductive outcome and antiphospholipid antibody-mediated pregnancy pathology in mice Mol. Hum. Reprod., June 1, 2004; 10(6): 409 - 416. [Abstract] [Full Text] [PDF]

				M. A. Ozturk, I. C. Haznedaroglu, M. Turgut, and H. Goker Current Debates in Antiphospholipid Syndrome: The Acquired Antibody-Mediated Thrombophilia Clinical and Applied Thrombosis/Hemostasis, April 1, 2004; 10(2): 89 - 126. [Abstract] [PDF]

				J. G. Hanly Antiphospholipid syndrome: an overview Can. Med. Assoc. J., June 24, 2003; 168(13): 1675 - 1682. [Abstract] [Full Text] [PDF]

				R A Asherson and R Cervera Antiphospholipid antibodies and infections Ann Rheum Dis, May 1, 2003; 62(5): 388 - 393. [Abstract] [Full Text] [PDF]

				G. M. Iverson, S. Reddel, E. J. Victoria, K. A. Cockerill, Y.-X. Wang, M. A. Marti-Renom, A. Sali, D. M. Marquis, S. A. Krilis, and M. D. Linnik Use of Single Point Mutations in Domain I of {beta}2-Glycoprotein I to Determine Fine Antigenic Specificity of Antiphospholipid Autoantibodies J. Immunol., December 15, 2002; 169(12): 7097 - 7103. [Abstract] [Full Text] [PDF]

				J.-C. Wasmuth, D. Oliver y Minarro, A. Homrighausen, L. Leifeld, J. K. Rockstroh, T. Sauerbruch, and U. Spengler Phospholipid Autoantibodies and the Antiphospholipid Antibody Syndrome: Diagnostic Accuracy of 23 Methods Studied by Variation in ROC Curves with Number of Clinical Manifestations Clin. Chem., July 1, 2002; 48(7): 1004 - 1010. [Abstract] [Full Text] [PDF]

				J. S. Levine, D. W. Branch, and J. Rauch The Antiphospholipid Syndrome N. Engl. J. Med., March 7, 2002; 346(10): 752 - 763. [Full Text] [PDF]

				T.-H. Luong, J. H. Rand, X.-X. Wu, J. H. Godbold, M. Gascon-Lema, and S. Tuhrim Seasonal Distribution of Antiphospholipid Antibodies Stroke, August 1, 2001; 32(8): 1707 - 1711. [Abstract] [Full Text] [PDF]

				H Guglielmone, S Vitozzi, O Elbarcha, and E Fernandez Cofactor dependence and isotype distribution of anticardiolipin antibodies in viral infections Ann Rheum Dis, May 1, 2001; 60(5): 500 - 504. [Abstract] [Full Text] [PDF]

				T. Avcin, A. Ambrozic, M. Kuhar, T. Kveder, and B. Rozman Anticardiolipin and anti-{beta}2 glycoprotein I antibodies in sera of 61 apparently healthy children at regular preventive visits Rheumatology, May 1, 2001; 40(5): 565 - 573. [Abstract] [Full Text] [PDF]

				A. Bili, A. J. Moss, C. W. Francis, W. Zareba, L. F. M. Watelet, I. Sanz, f. t. T. Factors, and R. C. E. Investigators Anticardiolipin Antibodies and Recurrent Coronary Events : A Prospective Study of 1150 Patients Circulation, September 12, 2000; 102(11): 1258 - 1263. [Abstract] [Full Text] [PDF]

				K. Ma, R. Simantov, J.-C. Zhang, R. Silverstein, K. A. Hajjar, and K. R. McCrae High Affinity Binding of beta 2-Glycoprotein I to Human Endothelial Cells Is Mediated by Annexin II J. Biol. Chem., May 12, 2000; 275(20): 15541 - 15548. [Abstract] [Full Text] [PDF]

				J G Hanly and S A Smith Autoimmune antiphospholipid antibodies and cryoglobulinemia Lupus, May 1, 2000; 9(4): 264 - 270. [Abstract] [PDF]

				J. Ordi-Ros, J. Villarreal, F. Monegal, S. Sauleda, I. Esteban, and M. Vilardell Anticardiolipin Antibodies in Patients with Chronic Hepatitis C Virus Infection: Characterization in Relation to Antiphospholipid Syndrome Clin. Vaccine Immunol., March 1, 2000; 7(2): 241 - 244. [Abstract] [Full Text] [PDF]

				E Matsuura and T Koike Accelerated atheroma and anti-{beta}2-glycoprotein I antibodies Lupus, March 1, 2000; 9(3): 210 - 216. [PDF]

				S. W. Reddel and S. A. Krilis Testing for and Clinical Significance of Anticardiolipin Antibodies Clin. Vaccine Immunol., November 1, 1999; 6(6): 775 - 782. [Full Text] [PDF]

				I Uthman, Z Tabbarah, and A E Gharavi Hughes syndrome associated with cytomegalovirus infection Lupus, November 1, 1999; 8(9): 775 - 777. [Abstract] [PDF]

				D Detkova, A Gil-Aguado, P Lavilla, M V Cuesta, G Fontaan, and D Pascual-Salcedo Do antibodies to {beta}2-glycoprotein 1 contribute to the better characterization of the antiphospholipid syndrome? Lupus, July 1, 1999; 8(6): 430 - 438. [Abstract] [PDF]

				V Guerin, A Ryman, and A Couchouron Transitory anti-{beta}2-glycoprotein I antibodies in infections Lupus, July 1, 1999; 8(6): 490 - 491. [PDF]

				S. Visvanathan and H. P. McNeil Cellular Immunity to {beta}2-Glycoprotein-1 in Patients with the Antiphospholipid Syndrome J. Immunol., June 1, 1999; 162(11): 6919 - 6925. [Abstract] [Full Text] [PDF]

				A Biasiolo, P Rampazzo, T Brocco, F Barbero, A Rosato, and V Pengo [Anti-{beta}2 Glycoprotein I--{beta}2 Glycoprotein I] immune complexes in patients with antiphospholipid syndrome and other autoimmune diseases Lupus, February 1, 1999; 8(2): 121 - 126. [Abstract] [PDF]

				Y Shoenfeld, A Gharavi, and T Koike b2GP-I in the anti phospholipid (Hughes') syndrome--from a cofactor to an autoantigen -- from induction to prevention of antiphospholipid syndrome Lupus, October 1, 1998; 7(8): 503 - 506. [PDF]

				D. Tanne, D. A. Triplett, and S. R. Levine Antiphospholipid-Protein Antibodies and Ischemic Stroke : Not Just Cardiolipin Any More Stroke, September 1, 1998; 29(9): 1755 - 1758. [Full Text] [PDF]

				N. Del Papa, Y. H. Sheng, E. Raschi, D. A. Kandiah, A. Tincani, M. A. Khamashta, T. Atsumi, G. R. V. Hughes, K. Ichikawa, T. Koike, et al. Human {beta}2-Glycoprotein I Binds to Endothelial Cells Through a Cluster of Lysine Residues That Are Critical for Anionic Phospholipid Binding and Offers Epitopes for Anti-{beta}2-Glycoprotein I Antibodies J. Immunol., June 1, 1998; 160(11): 5572 - 5578. [Abstract] [Full Text] [PDF]

				Y. Sheng, D. Kandiah, and S. Krilis Review : {beta} 2-Glycoprotein I : Target antigen for 'antiphospholipid' antibodies. Immunological and molecular aspects Lupus, January 1, 1998; 7(2_suppl): S5 - S9. [Abstract] [PDF]

				M. Kamboh and H. Mehdi Review : Genetics of apolipoprotein H ({beta}2-glycoprotein I) and anionic phospholipid binding Lupus, January 1, 1998; 7(2_suppl): S10 - S13. [Abstract] [PDF]

				J. Arnout and J. Vermylen Review : Mechanism of action of {beta}2-glycoprotein I-dependent lupus anticoagulants Lupus, January 1, 1998; 7(2_suppl): S23 - S28. [Abstract] [PDF]

				W. Wilson, Z. Faghiri, F. Taheri, and A. Gharavi Review : Significance of IgA antiphospholipid antibodies Lupus, January 1, 1998; 7(2_suppl): S110 - S113. [Abstract] [PDF]

				R. Brey and M. Carolin Detection of cerebral microembolic signals by transcranial Doppler may be a useful part of the equation in determining stroke risk in patients with antiphospholipid antibody syndrome Lupus, January 1, 1997; 6(8): 621 - 624. [PDF]

				S. Krilis, Y. Sheng, and D. Kandiah The role of {beta}2-glycoprotein I in the antiphospholipid syndrome Lupus, April 1, 1996; 5(2): 150 - 152. [Abstract] [PDF]

				M. Le Tonqueze, K. Salozhin, M. Dueymes, J.-C. Piette, V. Kovalev, Y. Shoenfeld, E. Nassonov, and P. Youinou Role of {beta}2-glycoprotein I in the antiphospholipid antibody binding to endothelial cells Lupus, June 1, 1995; 4(3): 179 - 186. [Abstract] [PDF]

				G. Balestrieri, A. Tincani, L. Spatola, F. Allegri, E. Prati, R. Cattaneo, G. Valesini, N. Del Papa, and P. Meroni Anti-beta 2-glycoprotein I antibodies: a marker of antiphospholipid syndrome? Lupus, April 1, 1995; 4(2): 122 - 130. [Abstract] [PDF]

				W. A Wilson, O. St. C Morgan, E. Barton, M. Smikle, B. Hanchard, W. Blattner, S. Doggett, and A. E Gharavi IgA antiphospholipid antibodies in HTLV-1-associated tropical spastic paraparesis Lupus, April 1, 1995; 4(2): 138 - 141. [Abstract] [PDF]

				O. Vaarala, M. Manttari, V. Manninen, L. Tenkanen, M. Puurunen, K. Aho, and T. Palosuo Anti-Cardiolipin Antibodies and Risk of Myocardial Infarction in a Prospective Cohort of Middle-Aged Men Circulation, January 1, 1995; 91(1): 23 - 27. [Abstract] [Full Text]

				M. Hojnik, B. Gilburd, L. Ziporen, M. Blank, Y. Tomer, M. A. Scheinberg, A. Tincani, B. Rozman, and Y. Shoenfeld Anticardiolipin Antibodies in Infections are Heterogenous in Their Dependency on {beta} 2-Glycoprotein I: Analysis of Anticardiolipin Antibodies in Leprosy Lupus, December 1, 1994; 3(6): 515 - 521. [Abstract] [PDF]

				E. Matsuura, M. Igarashi, Y. Igarashi, T. Katahira, H. Nagae, K. Ichikawa, D. A Triplett, and T. Koike Molecular studies on phospholipid-binding sites and cryptic epitopes appearing on {beta} 2-glycoprotein I structure recognized by anticardiolipin antibodies Lupus, July 1, 1994; 4(1_suppl): S13 - S17. [PDF]

				P. G. Vlachoyiannopoulos, E. Tsiakou, G. Chalevelakis, S. A. Raptis, and H. M. Moutsopoulos Antiphospholipid Syndrome: Clinical and Therapeutic Aspects Lupus, April 1, 1994; 3(2): 91 - 96. [Abstract] [PDF]

				M. D. Lockshin Antiphospholipid Antibody Syndrome JAMA, September 16, 1992; 268(11): 1451 - 1453. [Abstract] [PDF]