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Monoclonal Autoantibodies to Cardiolipin Derived from SLE Mice

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Takahiro Suzuki

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Yoshiko Hashimoto

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Takayuki Sumida

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Hisao Tomioka

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Sho Yoshida

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

Takao Koike

Second Department of Internal Medicine, Chiba University School of Medicine, 1-8-1 Inohana, Chiba 280, Japan

The objective of this study was to clarify the specificity of anticardiolipin antibodies (aCL). Eighteen monoclonal hybridoma aCL from systemic lupus erythematosus (SLE)-prone MRL/Mp-lpr/lpr mice were established, and the reactivity of monoclonal aCL to phospholipids, DNA, nuclei of human epithelial cells, platelets, vascular endothelial cells, heparin, protein C and thrombomodulin was examined.

All the 18 monoclonal aCL reacted with phosphatidylserine and some showed reactivity to phosphatidylinositol and phosphatidylcholine. Six of 16 monoclonal aCL were demonstrated to have the property of lupus anticoagulant. Monoclonal aCL were classified into three categories, in terms of DNA-binding specificity. Ten of 18 aCL had characteristics of antinuclear antibodies. Six of 11 aCL reacted with platelets. Three of 18 aCL were bound to vascular endothelial cells and to heparin. No monoclonal aCL reacted with protein C or thrombomodulin. Therefore, the conclusion was made that monoclonal aCL from SLE mice showed a polyspecific nature.

Key Words: Anticardiolipin antibody • Lupus anticoagulant • Anti-DNA antibody • Antiendothelial cell antibody • Antinuclear antibody

Lupus, Vol. 1, No. 4, 239-247 (1992)
DOI: 10.1177/096120339200100407


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