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Lupus
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Lack of Specific Binding of Anticardiolipin Antibodies to Intact Platelets

Hans-Jacob Haga

Lupus Arthritis Research Unit, The Rayne Institute, St Thomas' Hospital, London SE1 7EH

Costas Christopoulos

Haematology Department, University College and Middlesex School of Medicine, London W1, UK

Samuel J. Machin

Haematology Department, University College and Middlesex School of Medicine, London W1, UK

Munther Khamashta

Lupus Arthritis Research Unit, The Rayne Institute, St Thomas' Hospital, London SE1 7EH

Graham R.V. Hughes

Lupus Arthritis Research Unit, The Rayne Institute, St Thomas' Hospital, London SE1 7EH

The aims of this study were to investigate whether anticardiolipin antibodies (aCL) bind to intact (resting or activated) platelets in vitro. Suspensions of resting, activated (with a mixture of thrombin and collagen) and freeze-thawed platelets from healthy subjects were incubated with either affinity-purified aCL or pooled normal human immunoglobulin G (IgG). Platelet-bound IgG was measured by flow cytometric analysis of platelets incubated with a fluorescein-conjugated polyclonal goat anti-human IgG.

There was no significant binding of IgG aCL to intact resting or activated platelets, while significant specific binding to freeze-thawed platelets was demonstrated. These results question the theory that aCL bind/activate intact platelets in vivo.

Key Words: Anticardiolipin antibodies • Platelets • Flow cytometry

Lupus, Vol. 1, No. 6, 387-390 (1992)
DOI: 10.1177/096120339200100608
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