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In vitro ultraviolet irradiation induces pro-inflammatory responses in cells from premorbid SLE mice

Department of Immunology, Faculty of Medicine Technion and Divisionof Clinical Immunology, Bnai Zion Medical Center, Haifa, Israel; Department of Immunology, Faculty of Medicine, Technion, POB 9649,Haifa IL-31096, Israel.Tel: / 972 4 8295244; Fax: / 972 4 8295245 foltyn{at}techunix.technion.ac.il

T D Golan

Department of Immunology, Faculty of Medicine Technion and Divisionof Clinical Immunology, Bnai Zion Medical Center, Haifa, Israel

Systemic lupus erythematosus (SLE) is an autoimmune inflammatory disease, in which sunlight (especiallyits ultraviolet radiation (UVR)) is known to induce exacerbation of cutaneous lesions as well as systemic manifestations of the disease. The aim of this in vitro study was toinvestigate whether UVR (UVA, UVB) amplifies pro-inflammatory factors in cultured dermal fibroblasts (DF) or lymph node cells derived from premorbid or morbid mice from the murine SLE strains (MRL-1pr=1pr, (NZB/NZW)F1), in comparison to cells derived from normalmice from the non-SLE strains (C57BL=6, BALB/c). Our results demonstrate the following. Dermal fibroblast of premorbid SLE mice showed increased susceptibility toUVA and UVB irradiation, determined by viability assay, incomparison to those of normal mice. UVB irradiation inducedan enhanced expression of ICAM-1 in such SLE derived cells,in comparison to cells of normal mice. UVA and UVB increased functional activity of LFA-1 in lymph node cells of premorbid SLE mice and not in normal controls. UVB irradiation induced increased production and secretion of pro-inflammaory cytokines (IL-1, IL-6, TNF-a) in DF o premorbid SLE mice, in comparison to normal controls. Theenhanced pro-inflammatory responses to UVR werealsoobserved in experiments conducted with cells derivedfrom morbid SLE mice.

In conclusion, the pro-inflammatory pronenessdetected in the premorbid stage of murine SLE could be ofmajor importance in SLE pathogenesis. Furthermore, it suggests that the autoimmine inflammatory process in vivo, triggered initially by immune complex deposition, could be further amplified by UVR.

Key Words: SLE mice • UV irradiation • adhesion molecules • cytokines

Lupus, Vol. 10, No. 4, 272-283 (2001)
DOI: 10.1191/096120301680416968


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