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Hyperactivity in a mouse model of the antiphospholipid syndrome

Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv, Israel

C G Pick

Department of Anatomy, Sackler Faculty of Medicine, Tel Aviv, Israel

A D Korczyn

Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv, Israel; Department of Neurology, Sackler Faculty of Medicine, Tel Aviv, Israel

E Oest

Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv, Israel

M Blank

Department of Medicine B and Research Unit of Autoimmune Diseases, Sheba Medical Center, Tel Hashomer, Sackler Faculty of Medicine, Tel Aviv University, Israel

Y Shoenfeld

Department of Biochemistry, Sackler Faculty of Medicine, Tel Aviv, Israel

J Chapman

Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel jchapman{at}post.tau.ac.il; Department of Neurology, Sackler Faculty of Medicine, Tel Aviv, Israel; Department of Biochemistry, Sackler Faculty of Medicine, Tel Aviv, Israel

In the antiphospholipid syndrome (APS), antibodies to a complex of phospholipids and beta2-glycoprotein I (ß₂-GPI) are associated with recurrent thromboembolic events, spontaneous abortions, thrombocytopenia and central nervous system (CNS) disturbances. Animals immunized with b2-GPI develop the systemic manifestations of APS but the involvement of the (CNS) in these animals has not been studied. The objective of the present study was to examine mice with induced experimental APS for behavioral changes. Female Balb/C mice were immunized once with ß₂-GPI in complete Freund's adjuvant (CFA) or with CFA alone. Four months after immunization the mice were tested in the staircase apparatus and the following two variables were measured: (1) number of rears; and (2) number of stairs climbed by the mice. Immunization with ß₂-GPI resulted in elevated levels of circulating anti-negatively charged phospholipids and anti-ß₂-GPI antibodies. The APS mice exhibited hyperactive behavior as reflected by more frequent rears (P < 0.023) and higher number of stairs climbed (P < 0.019) by the mice in 3 min. This simple test demonstrated that experimental APS animals are significantly hyperactive and may serve as a marker for CNS involvement in this model.

Key Words: antiphospholipid syndrome • ß2-glycoprotein-I (apolipoprotein H) • animal model • staircase test • behavior

Lupus, Vol. 10, No. 7, 496-499 (2001)
DOI: 10.1191/096120301678416060


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