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The autoimmune response to chromatin antigens in systemic lupus erythematosus: autoantibodies against histone H1 are a highly specific marker for SLE associated with increased disease activity

Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Vienna, Austria

J Smolen

Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Vienna, Austria; 2nd Department of Medicine, Lainz Hospital, Vienna, Austria; Ludwig Boltzmann-Institute for Rheumatology and Balneology, Vienna, Austria

C Zimmermann

2nd Department of Medicine, Lainz Hospital, Vienna, Austria

H Hiesberger

Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Vienna, Austria

E Hoefler

2nd Department of Medicine, Lainz Hospital, Vienna, Austria

S Fournel

S Muller

Institute of Molecular and Cellular Biology, CNRS, Strasbourg, France

R L Rubin

Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA

G Steiner

Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Vienna, Austria; Ludwig Boltzmann-Institute for Rheumatology and Balneology, Vienna, Austria; Division of Rheumatology, Department of Internal Medicine III, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria guenter.steiner{at}akh-wien.ac.at

This study investigates specificity, sensitivity and concomitant presence of antibodies against histone H1 (H1), nucleosomes (NUC), chromatin (CHR) and dsDNA in patients with systemic lupus erythematosus (SLE), analyses their association with SLE disease activity and characterizes the immunodominant epitope reactivity of anti-H1 antibodies and its relation to SLE disease activity. In a cross-sectional study 394 sera of patients with various rheumatic diseases and healthy subjects were analysed by ELISA for antibodies against H1, NUC, CHR and dsDNA. In addition, a longitudinal analysis was performed that included 121 sequential serum samples derived from 16 SLE patients to assess the relation of these antibodies as well as antibodies to histone H2B to SLE disease activity. To assess epitope reactivity of anti-H1 antibodies overlapping synthetic peptides covering the entire H1 sequence were used. Anti-H1 antibodies yielded a sensitivity of approximately 45% and a specificity of over 98% for SLE, which was comparable to that found for anti-dsDNA antibodies. Anti-CHR and anti-NUC antibodies were of similar sensitivity but slightly (anti-CHR) or considerably (anti-NUC) less specific for SLE (95 and 85%, respectively). The sequential analysis revealed a strong correlation of anti-H1 antibodies with SLE disease activity that was better than the correlation of anti-dsDNA and anti-NUC antibodies, while only weak correlation was found for anti-CHR and anti-H2B antibodies. The immunodominant epitope for anti-H1 was localised between amino acids 204 and 218 (pp204–218) and immune reactivity to this epitope also correlated with disease activity. Anti-H1 is a highly specific marker for SLE with a diagnostic value comparable to anti-dsDNA. A positive testing for anti-H1 indicates increased disease activity, as does the appearance of antibodies to its immunodominant epitope pp204–218.

Key Words: autoantibodies • chromatin • disease activity • histone H1 • nucleosomes

Lupus, Vol. 11, No. 11, 704-715 (2002)
DOI: 10.1191/0961203302lu247oa


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