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Molecular expression systems for anti-DNA antibodies—1

Centre for Rheumatology, Department of Medicine, University College London, Arthur Stanley House, 40–50 Tottenham Street, London W1T 4NJ, UK anisur.rahman{at}ucl.ac.uk

J Haley

D S Latchman

Centre for Rheumatology, Department of Medicine, University College London, London, UK

Molecular expression systems can be used to produce whole antibodies or antibody fragments. The properties of these expression products can be tested in assays of binding or pathogenicity. Expression systems can be used to produce large quantities of antibodies which are already well-characterized,to produce new antibodies by repertoire cloning, or to produce slight modifications in the sequences of antibodies by mutagenesis prior to expression. This paper reviews the ways in which these methods have been used to study the structure and function of human and murine anti-DNA antibodies. A consistent finding, from experiments using a range of different expression methods and antibodies, is that sequence motifs including arginine residues play a major role in binding to DNA. These motifs can be present on either the heavy or the light chain, but are particularly reported in V_HCDR3.

Key Words: anti-DNA antibody expression system • arginine

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