This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Anand, A Articles by Lydyard, P M Search for Related Content PubMed PubMed Citation Articles by Anand, A Articles by Lydyard, P M Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Lupus Social Bookmarking                   What's this?

Characterization of CD3+CD4-CD8- (double negative) T cells in patients with systemic lupus erythematosus: activation markers

Department of Immunology and Molecular Pathology, Royal Free and University College School of Medicine, London, UK

G S Dean

Department of Immunology and Molecular Pathology, Royal Free and University College School of Medicine, London, UK; Centre for Rheumatology, Department of Medicine, Royal Free and University College School of Medicine, London, UK

K Quereshi

Department of Immunology and Molecular Pathology, Royal Free and University College School of Medicine, London, UK

D A Isenberg

Centre for Rheumatology, Department of Medicine, Royal Free and University College School of Medicine, London, UK

P M Lydyard

Department of Immunology and Molecular Pathology, Royal Free and University College School of Medicine, London, UK; Department of Immunology and Molecular Pathology, Royal Free and University College School of Medicine, Windeyer Research Institute, 46 Cleveland St, London W1T 4JF, UKp.lydyard{at}ucl.ac.uk

Systemic lupus erythematosus (SLE) is characterized by B cell hyperactivity and the production of autoantibodies, some of which (antibodies to dsDNA) are thought to be pathogenic. T helper cells drive the production of autoantibodies and the aim of this study is to characterize phenotypically a subpopulation of T cells (the CD3⁺CD4^-CD8^-, double negative (DN) T cells) previously identified as helping to enhance anti-DNA antibodies in patients with SLE. Data were obtained using FACS staining of DN T cells that had been purified from PBMCs by magnetic bead separation.

The percentage of TCR ab DN T cells was found to be significantly higher in patients with SLE as compared with controls (P ^ 0.02), although there was no significant increase in total percentage of DN T cells, which includes TCR gd cells. Activation markers HLA-DR and CD69, the costimulatory molecule CD28 and CTLA-4 were all expressed on the surface of a higher percentage of DN T cells in patients with SLE than in patients with rheumatoid arthritis (RA) or healthy controls (HC). More DN T cells from patients with SLE were of CD45RA phenotype than was found in controls, while CD45RO-expressing cells were reduced. In addition, DN T cells from patients with SLE expressed significantly higher levels of HLA-DR (P ^ 0.006), CD28 (P ^ 0.05), CTLA4 (P ^ 0.03) and CD45RA (P ^ 0.05) on the cell surface than those from the CD4=8 population. Correlation of expression of the markers measured with various parameters of disease activity and severity showed that high levels of HLA-DR expression correlated with high circulating serum C3 (> 0.9 IU/ml), indicating that an activated phenotype is consistent with severe disease.

Key Words: activation markers • double negative • SLE • T cells

Lupus, Vol. 11, No. 8, 493-500 (2002)
DOI: 10.1191/0961203302lu235oa


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. C. Crispin, M. Oukka, G. Bayliss, R. A. Cohen, C. A. Van Beek, I. E. Stillman, V. C. Kyttaris, Y.-T. Juang, and G. C. Tsokos Expanded Double Negative T Cells in Patients with Systemic Lupus Erythematosus Produce IL-17 and Infiltrate the Kidneys J. Immunol., December 15, 2008; 181(12): 8761 - 8766. [Abstract] [Full Text] [PDF]

				G S Dean, A Anand, A Blofeld, D A Isenberg, and P M Lydyard Characterization of CD3+CD4-CD8- (double negative) T cells in patients with systemic lupus erythematosus: production of IL-4 Lupus, August 1, 2002; 11(8): 501 - 507. [Abstract] [PDF]