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Association of homozygous deletion of the Humhv3005 and the VH3-30.3 genes with renal involvement in systemic lupus erythematosusDivision of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Department of Medicine, UCLA School of Medicine, Los Angeles, CA, USA
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea
Division of Rheumatology, Department of Internal Medicine, St Mary’s Hospital, and Research Institute of Immunobiology, Catholic Research Institutes of Medical Sciences, School of Medicine, The Catholic University of Korea, Seoul, Korea, chocs{at}cmc.cuk.ac.kr To investigate whether deletion of the Humhv3005 and the homologous VH3-30.3 (both share an identical amino acid sequence) genes is associated with susceptibility and/or certain clinical manifestations of systemic lupus erythematosus(SLE), DNA from 108 Korean SLE patients and 102 healthy subjects were analysed for the status of hv3005 gene by polymerase chain reaction-enzyme-linked immunosorbent assay. This method consists of amplification of selected germline VH3 genes with biotinylated primers, efficient capture of amplicons onto streptavidin-coated wells, and quantitative typing of bound VH3 gene with diagnostic oligonucleotides. We found that deletion of the hv3005 gene (including VH3-30.3) was more frequent in SLE patients than in healthy controls (26.9 versus 11.8%, P 0.006, odds ratio 2.75). When clinical features were examined, patients with hv3005 deletion have a higher frequency of lupus nephritis (LN) (75.9 versus 44.3% for those without, P 0.004), and higher activity index [median (range), 6 (2-14) versus 4 (1-16) for those without, P 0.044] when biopsy-provenLN was studied. Collectively, our data suggest that deletion of the hv3005 and the 3-30.3 genes may predispose individual SLE patients to the development of LN.
Key Words: Hv3005 gene • lupus nephritis • polymorphism • systemic lupus erythematosus
Lupus, Vol. 12, No. 5,
400-405 (2003) |
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