This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ornoy, A Articles by Shoenfeld, Y Search for Related Content PubMed PubMed Citation Articles by Ornoy, A Articles by Shoenfeld, Y Social Bookmarking                   What's this?

The effects of antiphospholipid antibodies obtained from women with SLE/APS and associated pregnancy loss on rat embryos and placental explants in culture

Laboratory of Teratology, Department of Anatomy and Cell Biology, Hebrew University Hadassah Medical School, Jerusalem, Israel, ornoy{at}cc.huji.ac.il

S Yacobi

Laboratory of Teratology, Department of Anatomy and Cell Biology, Hebrew University Hadassah Medical School, Jerusalem, Israel

S Tartakover Matalon

Laboratory of Teratology, Department of Anatomy and Cell Biology, Hebrew University Hadassah Medical School, Jerusalem, Israel, Research Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel

M Blank

Research Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel, Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

Z Blumenfeld

Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

R K Miller

Department of Obstetrics and Gynecology, Rochester University, Rochester, NY, USA

Y Shoenfeld

Research Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel

Recurrent fetal loss occurs in approximately 1% of women. Autoimmune causes have been suggested as a factor in some of these cases. High rates of intrauterine fetal growth retardation and increased incidence of prematurity is associated with systemic lupus erythematosus (SLE) and the antiphospholipid syndrome (APS). We found in previous studies that sera from SLE/APS patients when used as a culture medium for rat embryos were found to reduce embryonic growth and development, induce a high rate of embryonic anomalies and death and damage the yolk sac morphologicallyand functionally. In order to investigatethe direct effect of IgG purified from women with SLE/APS on the growth and viability of embryos, we cultured 11.5-day-old rat embryos in their yolk sacs in the presence of IgG purified from SLE/APS patients with recurrent pregnancy loss (RPL). The IgG affected directly the embryo and yolk sac, reducing their growth. The purified IgG positive for anticardiolipin/anti-DNA antibodies reduced yolk sac and embryonic growth more than sera negative for these antibodies but positive for antiphosphatydilserine and for antilaminin. Monoclonal antiphosphatydilserine reduced yolk sac growth but the embryos remained intact. Following the observed damage to the yolk sac we cultured human placental explants at 5.5-8 weeks of pregnancy in sera from SLE/APS patients for 96 hours and found that these sera reduced placental trophoblastic cell growth, reduced their proliferation rate and increased their rate of apoptosis. Successful treatment of the women resulted in a correction of the damage induced in the cultured rat embryos and in the cultured placental explants.

Key Words: embryo • placental explants • pregnancy loss • SLE/APS

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. DI SIMONE, M. P. LUIGI, D. MARCO, D. N. FIORELLA, D. SILVIA, D. M. CLARA, and C. ALESSANDRO Pregnancies Complicated with Antiphospholipid Syndrome: The Pathogenic Mechanism of Antiphospholipid Antibodies: A Review of the Literature Ann. N.Y. Acad. Sci., June 1, 2007; 1108(1): 505 - 514. [Abstract] [Full Text] [PDF]

				N. DI SIMONE, R. CASTELLANI, E. RASCHI, M. ORIETTA BORGHI, P. L. MERONI, and A. CARUSO Anti-Beta-2 Glycoprotein I Antibodies Affect Bcl-2 and Bax Trophoblast Expression without Evidence of Apoptosis Ann. N.Y. Acad. Sci., June 1, 2006; 1069(1): 364 - 376. [Abstract] [Full Text] [PDF]

				P L Meroni, N di Simone, C Testoni, M D'Asta, B Acaia, and A Caruso Antiphospholipid antibodies as cause of pregnancy loss Lupus, September 1, 2004; 13(9): 649 - 652. [Abstract] [PDF]