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Telomerase activity and telomere length of peripheral blood mononuclear cells in SLE patients

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan, Department of Molecular Immunology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo, Japan, d_kurosaka{at}jikei.ac.jp

Jun Yasuda

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan, Department of Molecular Immunology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo, Japan

Ken Yoshida

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan, Department of Molecular Immunology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo, Japan

Toru Yokoyama

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

Yoshinori Ozawa

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

Yutaka Obayashi

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

Isamu Kingetsu

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

Saburo Saito

Department of Molecular Immunology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo, Japan

Akio Yamada

Division of Rheumatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

We evaluated the clinical significance of the telomerase activity and telomere length of peripheral blood mononuclearcells (PBMC) in systemic lupus erythematosus (SLE). PBMC were isolated from 55 patients with SLE and the telomerase activity was measured by TRAP assay. The telomere length of PBMC was also measured in 30 of these subjects. As a control group, 45 healthy adults with no particular clinical history were studied. The results were compared with clinical data. In patients with active SLE, the telomerase activity of PBMC was significantly increased compared with the control group. In patients with inactive SLE, the PBMC telomerase activity was not different compared with the controls in their 20s, 30s and 40s, but it was significantly increased compared with the controls in their 50s. In SLE patients, the telomerase activity of PBMC was significantly correlatedwith modified SLEDAI. The telomere length of PBMC in younger SLE patients tended to be shorter than that in the controls, but no difference was observed in older patients. The correlation coefficient between the telomerase activity and telomere length of PBMC in SLE patients was not significant. Abnormalities in the telomeraseactivity and telomere length observedin SLE patients are consideredto be important findings for evaluation of the pathology of SLE.

Key Words: age • SLEDAI • systemic lupus erythematosus • telomerase • telomere

Lupus, Vol. 12, No. 8, 591-599 (2003)
DOI: 10.1191/0961203303lu426oa


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