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Lupus, Vol. 13, No. 1, 24-31 (2004)
DOI: 10.1191/0961203304lu488oa

Expression and activity analyses of CTLA4 in peripheral blood lymphocytes in systemic lupus erythematosus patients

M Hirashima

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan, jmika{at}med.juntendo.ac.jp

T Fukazawa

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

K Abe

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

Y Morita

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

M Kusaoi

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

H Hashimoto

Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan

The objective of this study was to determine the expression and activity of CTLA4 in T-cells of systemic lupus erythematosus (SLE) patients. Expression of CTLA4 on freshly isolated peripheral blood T-cells was evaluated in 33 SLE patients and 25 controls using flow cytometry.The T-cells from 19 SLE patients and 22 controls were stimulated and cultured with Chinese hamster ovary cells expressing CD80 (CHO-CD80) or with CHO cells. T-cell proliferation was determined with [3H] thymidine incorporation (CPM), and the inhibitory effect of CTLA4 on T-cell proliferation was evaluated by the ratio of CPM for T-cells with CHO -CD80 cells to that of T-cells with CHO cells (the CHO -CD80/CHO ratio). IntracellularCTLA4 expressionin freshly isolated peripheral blood T-cells was significantly higher in SLE patients than the controls (P < 0.05), but there was no correlation with clinical features or disease activity. The CHO -CD80/CHO ratio of SLE patients was significantly higher than that of the controls(P < 0.05). Among SLE patients, the CHO -CD80/CHO ratio of patients with lupus nephritis was significantly higher than that of patients without lupus nephritis (P < 0.05). In conclusion, our data suggest that CTLA4 expression is not impaired in SLE patients, but there is a possibility of decreased inhibitory effect of CTLA4 involved in the pathogenesis of SLE.

Key Words: CTLA4 • lupus nephritis • systemic lupus erythematosus


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