This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ames, P R. Articles by Brancaccio, V Search for Related Content PubMed PubMed Citation Articles by Ames, P R. Articles by Brancaccio, V Social Bookmarking                         What's this?

Lymphocyte subpopulations and intima media thickness in primary antiphospholipd syndrome

Academic Department of Rheumatology, Leeds General Infirmary, Leeds, UK, paxmes{at}aol.com

C Tommasino

Chemical Pathology and Immunology San Gennaro Hospital, Naples, Italy

G Fossati

Chemical Pathology and Immunology San Gennaro Hospital, Naples, Italy

E Matsuura

Department of Biochemistry, Okyama University, Okayama, Japan

A Margarita

Angiology and Coagulation Units, Cardarelli Hospital, Naples, Italy

A Saulino

Chemical Pathology and Immunology San Gennaro Hospital, Naples, Italy

L Lopez

Corgenix, Westminster, USA

G Scenna

Corgenix, Westminster, USA

V Brancaccio

Angiology and Coagulation Units, Cardarelli Hospital, Naples, Italy

The aim of this study was to evaluate a possible association between lymphocyte subsets and intima media thickness (IMT) of carotid arteries in primary antiphospholipid syndrome (PAPS). We used a cross-sectional study on PAPS patients (n = 18) and healthy controls (n = 16). IgG anti-cardiolipin antibody (aCL), IgG anti-ß₂glycoprotein-I (anti-ß₂GPI), IgG anti-ß₂glycoprotein-I complexed to oxidized low-density lipoprotein (oxLDL) and to a specific oxidized moiety of LDL (oxLig1), and ß₂GPI-oxLDL were measured by ELISA. Lymphocyte immunophenotyping was performed using pairs of monoclonal antibodies directly labelled with fluorescein isothiocyanate, or phycoerythrin or phycoerythrin-Texas-red-X. Intima media thickness (IMT) of carotid arteries was determined by high-resolution sonography. Total peripheral blood lymphocytes did not differ between PAPS and controls. Memory CD4+/CD45RO + T cells were lower in PAPS than controls (P = 0.0007) as well as CD16+56+ natural killer cells (P = 0.02). In PAPS memory T CD45RO + cells positively correlated with IgG anti-ß2GPI-oxLig1 (P = 0.002) and to IMT of carotid arteries (common carotid P = 0.02, bifurcation P = 0.007). Naïve CD4+/CD45RA+ T cells inversely correlated with ß2GPI-oxLDL (P = 0.009). The relation between IgG anti-ß2GPI-oxLig1 and IMT of carotid arteries with memory CD45RO + T lymphocytes suggests a role for the latter in PAPS related atherogenesis.

Key Words: antiphospholipid antibodies • atherosclerosis • IgG anti-ß2GPI-oxLig1 • lymphocytes

Lupus, Vol. 14, No. 10, 809-813 (2005)
DOI: 10.1191/0961203305lu2181oa


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. CARBONE, A. GALLEGO, N. LANIO, J. NAVARRO, M. ORERA, A. AGUARON, E. FERNANDEZ-CRUZ, and E. SARMIENTO Quantitative Abnormalities of Peripheral Blood Distinct T, B, and Natural Killer Cell Subsets and Clinical Findings in Obstetric Antiphospholipid Syndrome J Rheumatol, June 1, 2009; 36(6): 1217 - 1225. [Abstract] [Full Text] [PDF]