This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (1) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Li, X Articles by Abdou, N I Search for Related Content PubMed PubMed Citation Articles by Li, X Articles by Abdou, N I Social Bookmarking                         What's this?

Estrogen does not regulate CD154 mRNA stability in systemic lupus erythematosus T cells

Department of Biology, Pittsburg State University, Pittsburg, Kansas, USA, Department of Dermatology and Rheumatology, Kunming Medical College, Kunming, People’s Republic of China

V Rider

Department of Biology, Pittsburg State University, Pittsburg, Kansas, USA, vrider{at}pittstate.edu

B F Kimler

Department of Radiation Oncology, The University of Kansas Medical Center, Kansas City, Kansas, USA

N I Abdou

Center of Rheumatic Diseases, Allergy-Immunology, Kansas City, MO, USA

Previous studies in our laboratory showed a dose-dependent and hormone-specific increase in CD154 expression in T cells from females with systemic lupus erythematosus (SLE). This present study investigates if the estrogen-dependent increase in CD154 expression is due to stabilization of the messenger RNA. T cells from female SLE patients and controls were cultured for 18 h in serum-free medium without and with estradiol 17-ß (10⁷ M). T cells were either unstimulated (resting) or were activated by further culture on anti-CD3 coated plates. Actinomycin D (25 g/mL) was added to parallel cultures to inhibit new messenger RNA synthesis. CD154 messenger RNA stability was assessed by reverse-transcription polymerase chain amplification. Resting SLE (n = 10, P = 0.88) and normal (n = 7, P = 0.65) T cells showed no significant differences in message stability in response to estradiol. CD154 messenger RNA was also not significantly stabilized in activated SLE (n = 10, P = 0.15) or activated normal (n = 6, P = 0.077) T cells in response to estradiol. These findings indicate that the estrogen-dependent increase in CD154 in SLE T cells is not due to stability of the mRNA. These data are consistent with the postulate that estradiol stimulates CD154 transcription in SLE T cells.

Key Words: CD154 • estrogen • mRNA stability • SLE • T cell

Lupus, Vol. 15, No. 12, 852-857 (2006)
DOI: 10.1177/0961203306071314


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				L. Shao, H. Fujii, I. Colmegna, H. Oishi, J. J. Goronzy, and C. M. Weyand Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis J. Exp. Med., June 8, 2009; 206(6): 1435 - 1449. [Abstract] [Full Text] [PDF]