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Lupus, Vol. 15, No. 7, 436-441 (2006)
DOI: 10.1191/0961203306lu2330oa

Application of a Static Fluorescence-Based Cytometer: The Cellscan in Clinical Immunology

M Szyper-Kravitz

Center for Autoimmune Diseases, Department of Medicine B, Chaim Sheba Medical Center, Tel-Hashomer, Israel

M Harel

Center for Autoimmune Diseases, Department of Medicine B, Chaim Sheba Medical Center, Tel-Hashomer, Israel

B Gilburd

Center for Autoimmune Diseases, Department of Medicine B, Chaim Sheba Medical Center, Tel-Hashomer, Israel

E Trubniykov

Medis-El Ltd, Lod, Israel

YS Schiffenbauer

Medis-El Ltd, Lod, Israel

Y Shoenfeld

Center for Autoimmune Diseases, Department of Medicine B, Chaim Sheba Medical Center, Tel-Hashomer, Israel, Incumbent of the Laura Schwarz-Kipp Chair for Research of Autoimmune Diseases, Tel-Aviv University, Israel, shoenfel{at}post.tau.ac.il

The CellScan system is a laser scanning cytometer which enables repetitive fluorescence intensity (FI) and polarization (FP) measurements in living cells, as a means of monitoring lymphocyte activation. By monitoring FP changes in peripheral blood lymphocytes (PBL) following exposure to antigenic stimuli, the CellScan may have a role in the diagnosis of autoimmune diseases. Monitoring changes in FI and FP in PBLs from patients with atherosclerosis following exposure to various stimuli, has illustrated the role of the immune system in the atherosclerotic process. The CellScan has also been evaluated as a diagnostic tool for drug-induced allergy, based on FP reduction in PBLs following incubation with the suspected drugs. FI and FP changes in cancer cells have been found to correlate with the cytotoxic effect of different anti-neoplastic drugs, illustrating the potential role of the CellScan system in clinical oncology. In conclusion, the CellScan is a promising new tool with a variety of applications in cell biology, immunology, cancer research and clinical pharmacology.

Key Words: atherosclerosis • cancer • CellScan • drug allergy • fluorescence polarization • systemic lupus erythematosus • rheumatoid arthritis


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