This Article Free Full Text (Free PDF) Free References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Beier, F. Articles by Brummendorf, T.H. Search for Related Content PubMed PubMed Citation Articles by Beier, F. Articles by Brummendorf, T.H. Social Bookmarking                         What's this?

Telomere length analysis in monocytes and lymphocytes from patients with systemic lupus erythematosus using multi-color flow-FISH

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany

S. Balabanov

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Hematology and Oncology, University Hospital Eppendorf, Hamburg, Germany

C.C. Amberger

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany

U. Hartmann

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Pharmacology, University of Tubingen, Germany

K. Manger

Department of Rheumatology, University of Erlangen, Germany

K. Dietz

Department of Medical Biometry, University of Tubingen, Germany

I. Kötter

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany

T.H. Brummendorf

Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Hematology and Oncology, University Hospital Eppendorf, Hamburg, Germany, t.bruemmendorf{at}uke.uni-hamburg.de

In order to analyse telomere length in subsets of human peripheral blood lymphocytes and monocytes, we modified a recently developed multicolor flow- fluorescent in situ hybridization (FISH) methodology that combines flow-FISH and antibody staining for cell surface antigens. We analysed telomere length of peripheral blood mononuclear cells in a group of 22 patients with systemic lupus erythematosus (SLE) and 20 age-matched healthy donors. We found that neither CD4+, CD8+, CD19+ cells nor CD14+ monocytes have significantly shorter telomeres compared with their healthy counterparts. On the basis of these findings, we then used monocyte telomere length as internal reference in order to control for intra-individual variability in telomere length. By using this approach, we could demonstrate significant telomere shortening in all three lymphocyte subsets (in all cases P < 0.05) compared with monocytes. However, these differences did not vary significantly between SLE patients and controls. In summary, telomere lengths in subpopulations of hematopoietic cells can be monitored in patients with SLE using multicolor flow-FISH. While confirming data by other groups on telomere length in lymphocyte subpopulations, our data argue against an increased proliferation rate of peripheral blood monocytes reflected by accelerated telomere shortening in patients with SLE. Lupus (2007) 16, 955—962.

Key Words: flow-FISH • monocyte • systemic lupus erythematosus • telomere • telomerase

Lupus, Vol. 16, No. 12, 955-962 (2007)
DOI: 10.1177/0961203307084299


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?