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Expression of nucleic acid binding Toll-like receptors in control, lupus and transplanted kidneys – a preliminary pilot study

III. Department of Internal Medicine, Faculty of Medicine and Faculty Hospital, University of Olomouc, Olomouc, Czech Republic

P Horak

III. Department of Internal Medicine, Faculty of Medicine and Faculty Hospital, University of Olomouc, Olomouc, Czech Republic

YT Konttinen

Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland; ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland; Coxa Hospital for the Joint Replacement, Tampere, Finland

K Krejci

III. Department of Internal Medicine, Faculty of Medicine and Faculty Hospital, University of Olomouc, Olomouc, Czech Republic

T Tichy

Department of Pathology, Faculty of Medicine and Faculty Hospital, University of Olomouc, Olomouc, Czech Republic

Z Hermanova

Department of Clinical Immunology, Faculty Hospital, Olomouc, Czech Republic

J Zadrazil

III. Department of Internal Medicine, Faculty of Medicine and Faculty Hospital, University of Olomouc, Olomouc, Czech Republic

We hypothesized that nucleic acids, free and/or complexed, filtered and/or locally released, might be entrapped in the kidneys because of the specific nucleic acid binding microbial pattern recognizing Toll-like receptors (TLRs). This hypothesis of nucleic acid binding potential was tested using paraffin sections from healthy control, SLE and transplant kidneys, which were labelled using TLR-specific rabbit or goat anti–human antibodies in immunoperoxidase staining. Normal and transplant kidneys contain some double- (TLR-3) and single-stranded RNA binding (TLR-8) receptors, but in particular double-stranded RNA binding receptor TLR-7, mostly in tubuli, whereas no DNA binding TLR-9 was found. SLE kidneys contain more TLR-3 and TLR-8 and express de novo also TLR-9, in particular in glomeruli. On the contrary, TLR-7 was relatively weak in SLE. It is concluded that kidneys have a capacity to bind nucleic acids. TLR stimulation leads to the production of tumour necrosis factor- and other pro-inflammatory cytokines and to up-regulation of co-stimulatory molecules necessary for the adaptive immune response. This makes renal tissues a potential target for inflammatory and immune responses in autoimmune disease and in the reaction for the foreign tissue.

Key Words: lupus nephritis • transplanted kidney • TLR-3 • TLR-7 • TLR-8 • TLR-9

Lupus, Vol. 17, No. 6, 580-585 (2008)
DOI: 10.1177/0961203307088130


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