This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Dai, Y Articles by Lv, T Search for Related Content PubMed PubMed Citation Articles by Dai, Y Articles by Lv, T Social Bookmarking                   What's this?

A proteomic study of peripheral blood mononuclear cells in systemic lupus erythematosus

The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China xiaoyong62{at}tom.com

C Hu

The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China

Y Huang

Key laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing, China

HY Huang

Shenzhen Center for Disease Control and Prevention, Guangdong Province, China

J Liu

Shenzhen Center for Disease Control and Prevention, Guangdong Province, China

T Lv

The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Guangdong Province, China

Our objective was to analyze the changes in the protein expression profiles of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE). Peripheral blood was obtained from patients with SLE and healthy controls. 2-D gel electrophoresis was performed, and gels were silver-stained. Differentially expressed protein spots were detected, some of which were identified by MALDI-TOF spectrometry. Match rates of 71% ± 4% and 72% ± 4% were gotten for control and patient gels, respectively. 791 ± 17 spots were detected for control gels and 781 ± 17 for patient gels. Eleven protein spots were up-regulated, and 9 protein spots were down-regulated in patients with SLE. Five differentially expressed proteins were identified as immunoglobulin J chain, apolipoprotein A-IV precursor, calprotectin L1H and zinc finger protein subfamily 1A (all up-regulated) and glutathione S-transferase (down-regulated), some of which had previously been shown to play a potential role in the pathogenesis of SLE. We conclude there are significant changes in the 2-D maps of PBMCs in patients with SLE and applying this proteomic approach may be a useful way to gain novel insights into SLE.

Key Words: proteomics • systemic lupus erythematosus • 2-D gel electrophoresis

Lupus, Vol. 17, No. 9, 799-804 (2008)
DOI: 10.1177/0961203308089444


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Wu, Z. Huang, Y. Shi, B. Cai, L. Wang, B. Ying, C. Hu, Y. Li, and W. Liang Systemic Lupus Erythematosus (SLE) Risk Factors: Novel Proteins Detected From Familial SLE Using Proteomics Lab Med, July 1, 2009; 40(7): 408 - 411. [Abstract] [Full Text] [PDF]