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Response to ODN-CpG by B Cells from patients with systemic lupus erythematosus correlates with disease activity

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Immunology, Cancer Center, Scottsdale Mayo Clinic Arizona, Scottsdale, Arizona, USA

M Arias

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Department of Cellular and Molecular Medicine, St George’s University of London, London, UK

F Riaño

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

S Paris

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

LA Ramírez

Grupo de Reumatología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

O Uribe

Grupo de Reumatología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

LF García

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

G Vásquez

Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Grupo de Reumatología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombiaglomavas{at}gmail.com

Different immunological alterations may condition systemic lupus erythematosus (SLE) activity. However, it is not known whether alterations in the phenotype of circulating antigen-presenting cells (APCs) and in the response to CpG oligodeoxynucleotides (ODN-CpG) correlate with disease activity. APC expression of HLA-DR, costimulatory molecules, and TLR9 expression was determined in patients with SLE, other autoimmune diseases, and healthy controls. Monocyte and B cell response to synthetic ODN-CpG sequences was also evaluated. Monocytes from patients with moderate SLE activity had higher expression of CD40 and CD86. Decreased numbers of CD19+CD80+ and BDCA-3+CD40+ cells were found in patients with severe SLE activity. In patients with moderate SLE activity, non-adherent and enriched B cell response to ODN-CpG was similar to healthy controls. Adherent and enriched B cells from patients with severe SLE activity did not increase costimulatory molecule expression or cytokine production after ODN-CpG stimulation. APCs from patients with SLE, regardless of disease activity, displayed higher percentage of TLR9+ cells, as well as increased expression of TLR9, compared to healthy controls. Results suggest that the B cell response to ODN-CpG correlates with the SLE activity, independently of TLR9 expression, indicating that alterations in B cell response in severe activity SLE may be caused by events down-stream to TLR9.

Key Words: autoimmunity • CpG • SLE • TLR 9

Lupus, Vol. 18, No. 8, 718-726 (2009)
DOI: 10.1177/0961203309103098


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