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Anticardiolipin Antibodies in Infections are Heterogenous in Their Dependency on ß 2-Glycoprotein I: Analysis of Anticardiolipin Antibodies in Leprosy

Department of Rheumatology, University Medical Center, Ljubljana, Slovenia

Boris Gilburd

Research Unit of Autoimmune Diseases and the Department of Medicine 'B', Sheba Medical Center, Tel-Hashomer (affiliated to Tel-Aviv University Faculty of Medicine), Israel

Lea Ziporen

Research Unit of Autoimmune Diseases and the Department of Medicine 'B', Sheba Medical Center, Tel-Hashomer (affiliated to Tel-Aviv University Faculty of Medicine), Israel

Miri Blank

Research Unit of Autoimmune Diseases and the Department of Medicine 'B', Sheba Medical Center, Tel-Hashomer (affiliated to Tel-Aviv University Faculty of Medicine), Israel

Yaron Tomer

Research Unit of Autoimmune Diseases and the Department of Medicine 'B', Sheba Medical Center, Tel-Hashomer (affiliated to Tel-Aviv University Faculty of Medicine), Israel

Morton A. Scheinberg

Institute of Cancer 'Arnold Vieira de Carvalho', Division of Immunology, Sao Paulo, Brazil

Angela Tincani

Servizio di Immunologia Clinica, Brescia, Italy

Blaz Rozman

Department of Rheumatology, University Medical Center, Ljubljana, Slovenia

Yehuda Shoenfeld

Research Unit of Autoimmune Diseases and the Department of Medicine 'B', Sheba Medical Center, Tel-Hashomer (affiliated to Tel-Aviv University Faculty of Medicine), Israel

We studied the effect of ß ₂-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine ß₂-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without ß₂-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of ß₂-GPI on the leprosy and APS sera binding to CL was confirmed using purified human ß ₂-GPI. Enhanced binding was seen if ß₂-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against ß₂-GPI were found by ELISA. Leprosy anti-ß₂-GPI antibodies appear to be a separate antibody population recognizing only ß₂-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their ß ₂-GPI requirement for binding to CL.

Key Words: Leprosy • Anticardiolipin antibodies • Antiphospholipid antibodies • ß2-glycoprotein 1

Lupus, Vol. 3, No. 6, 515-521 (1994)
DOI: 10.1177/096120339400300615


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