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Age-related differential mRNA expression of T cell cytokines in NZB/NZW F1 mice

Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Department of Haematology and Immunology, Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo 673-14, Japan

K. Yamamoto

Division of Rheumatology and Molecular Immunology, Institute of Medical Science, St. Marianna University, School of Medicine. Kawasaki 216

H. Okudaira

Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113

M. Dohi

Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113

M. Suko

Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113

Y. Fukata

Department of Haematology and Immunology, Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo 673-14, Japan

H. Yago

Department of Haematology and Immunology, Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo 673-14, Japan

S. Suehiro

Department of Haematology and Immunology, Institute of Bio-Active Science, Nippon Zoki Pharmaceutical Company, Hyogo 673-14, Japan

K. Ito

Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113

The mRNA expression of interleukin (IL)-2, IL-2 receptor-alpha-chain (IL-2R, IL-4 and interferon-gamma (IFN-) in spleen cells from NZB/NZW F₁ (B/W F₁) mice following the stimulation with concanavalin A (Con A) was examined by Northern blot analysis. Kinetic patterns of the mRNA expression after the stimulation were not different between 2-month-old and 6 to 8-month-old B/W F₁ mice. However, relative mRNA expression of IL-2 to a cytoskeletal protein, -Tubulin was lower in 6 to 8-month-old B/W F, mice than in 2-month-old mice. Similar but not significant tendency was observed in IL-2R mRNA expression. In contrast, relative IL-4 mRNA expression in 6 to 8-month-old B/W F₁ mice was significantly higher than that in 2-month-old animals. On the other hand, no apparent change was observed in IFN- mRNA expression. Flow cytometric analysis indicated that there was no apparent difference in proportion of L3T4 positive T cells in spleen cells from 2 and 6 to 8-month-old B/W F₁ mice. These results suggest that mRNA expression of IL-2 and IL-4 differentially changes with aging in autoimmune B/W F₁ mice.

Key Words: cytokine • mRNA • NZB/NZW F1 mice • aging • Northern blotting

Lupus, Vol. 4, No. 3, 213-216 (1995)
DOI: 10.1177/096120339500400309


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