This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Williams, W.M. Articles by Isenberg, D.A. Search for Related Content PubMed PubMed Citation Articles by Williams, W.M. Articles by Isenberg, D.A. Social Bookmarking                         What's this?

Human T cell responses to autoantibody variable region peptides

Bloomsbury Rheumatology Unit/Division of Rheumatology, Arthur Stanley House, 40-50 Tottenham Street, London W1P 9PG

N.A. Staines

Infection and Immunity Research Group, Kings College London, London, UK

S. Muller

Molecular and Cellular Biology Institute, Strasbourg, France

D.A. Isenberg

The origins and regulation of autoantibodies in SLE may involve idiotypic cell interactions. The purpose of this study was to determine if SLE patients have T cells reactive with the idiotopes of autoantibodies. Sequences of the variable regions of two DNA-binding autoantibodies (V of antibody B3 and VH of 9G4) were selected according to the predicted location of their idiotypes defined previously by anti-idiotypic antibodies. The sequences were prepared as synthetic 16mer peptides (idiopeptides). Peripheral blood mononuclear cells were prepared from SLE patients (n = 28) and controls (n = 13) and put into multiple microcultures with idiopeptide for 6 days. The frequency of responding cultures was determined as those incorporating thymidine at levels above the mean plus three standard deviations of the control cultures lacking peptide. Of the 28 lupus patients, six responded to B3 idiopeptide and five to the 9G4 idiopeptide. Some patients responded to other idiopeptides, but only one normal individual responded to each reference peptide. The difference between the patient and control responses to all idiopeptides was significant by ² analysis (P = 0.025). We conclude that patients with SLE show evidence of sensitisation of T cells to idiotopes of autoantibodies. Such anti-idiotypic T cells could either provide idiotype-specific help or suppression for autoantibody responses in SLE.

Key Words: anti-DNA antibodies • T cells • peptide • idiotype

Lupus, Vol. 4, No. 6, 464-471 (1995)
DOI: 10.1177/096120339500400608


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Zangani, H. Carlsen, A. Kielland, A. Os, H. Hauglin, R. Blomhoff, L. A. Munthe, and B. Bogen Tracking Early Autoimmune Disease by Bioluminescent Imaging of NF-{kappa}B Activation Reveals Pathology in Multiple Organ Systems Am. J. Pathol., April 1, 2009; 174(4): 1358 - 1367. [Abstract] [Full Text] [PDF]

				B. C. Beaudette-Zlatanova, T. Ling, M. J. Shlomchik, A. Marshak-Rothstein, and I. R. Rifkin B Cells and Dendritic Cells from V{kappa}8 Light Chain Transgenic Mice Activate MRL-lpr/gld CD4+ T Cells J. Immunol., July 1, 2006; 177(1): 45 - 52. [Abstract] [Full Text] [PDF]

				W. Guo, D. Smith, A. Guth, K. Aviszus, and L. J. Wysocki T Cell Tolerance to Germline-Encoded Antibody Sequences in a Lupus-Prone Mouse J. Immunol., August 15, 2005; 175(4): 2184 - 2190. [Abstract] [Full Text] [PDF]

				L. A. Munthe, A. Corthay, A. Os, M. Zangani, and B. Bogen Systemic Autoimmune Disease Caused by Autoreactive B Cells That Receive Chronic Help from Ig V Region-Specific T Cells J. Immunol., August 15, 2005; 175(4): 2391 - 2400. [Abstract] [Full Text] [PDF]

				J K Kalsi, J Grossman, J Kim, P Sieling, D W Gjertson, E F Reed, F M Ebling, M Linker-Israeli, and B H Hahn Peptides from antibodies to DNA elicit cytokine release from peripheral blood mononuclear cells of patients with systemic lupus erythematosus: relation of cytokine pattern to disease duration Lupus, July 1, 2004; 13(7): 490 - 500. [Abstract] [PDF]

				L. A. Munthe, A. Os, M. Zangani, and B. Bogen MHC-Restricted Ig V Region-Driven T-B Lymphocyte Collaboration: B Cell Receptor Ligation Facilitates Switch to IgG Production J. Immunol., June 15, 2004; 172(12): 7476 - 7484. [Abstract] [Full Text] [PDF]

				J K Kalsi and B H Hahn The role of VH determinants in systemic lupus erythematosus Lupus, December 1, 2002; 11(12): 878 - 884. [Abstract] [PDF]

				R. R. Singh, F. M. Ebling, D. A. Albuquerque, V. Saxena, V. Kumar, E. H. Giannini, T. N. Marion, F. D. Finkelman, and B. H. Hahn Induction of Autoantibody Production Is Limited in Nonautoimmune Mice J. Immunol., July 1, 2002; 169(1): 587 - 594. [Abstract] [Full Text] [PDF]

				M. L. Stoll and J. Gavalchin Systemic lupus erythematosus--messages from experimental models Rheumatology, January 1, 2000; 39(1): 18 - 27. [Full Text] [PDF]

				P. Hobby, F. J. Ward, A. N. Denbury, D. G. Williams, N. A. Staines, and B. J. Sutton Molecular Modeling of an Anti-DNA Autoantibody (V-88) and Mapping of Its V Region Epitopes Recognized by Heterologous and Autoimmune Antibodies J. Immunol., September 15, 1998; 161(6): 2944 - 2952. [Abstract] [Full Text] [PDF]

				B. H. Hahn Antibodies to DNA N. Engl. J. Med., May 7, 1998; 338(19): 1359 - 1368. [Full Text] [PDF]

				J. Gavalchin and N.A. Staines T and B cell recognition of idiotypes of anti-DNA autoantibodies Lupus, January 1, 1997; 6(3): 337 - 343. [PDF]