SAGE Journals Online
Advertisement
Sign In to gain access to subscriptions and/or personal tools.

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Advertisement

Sign In to gain access to subscriptions and/or personal tools.
Lupus
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Le Tonquèze, M.
Right arrow Articles by Youinou, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Tonquèze, M.
Right arrow Articles by Youinou, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Establishment and characterization of permanent human endothelial cell clones

M. Le Tonquèze

Laboratory of Immunology, Brest University Medical School Hospital, Brest, France

C. Jamin

Laboratory of Immunology, Brest University Medical School Hospital, Brest, France

M. Böhme

Medizinische Klinik, Ruprecht-Karls Universität, Heidelberg, Germany

R. Le Corre

Laboratory of Immunology, Brest University Medical School Hospital, Brest, France

M. Dueymes

Laboratory of Immunology, Brest University Medical School Hospital, Brest, France

P. Youinou

Laboratory of Immunology, Brest University Medical School Hospital, Brest, France

Though vasculitic diseases have been claimed to be associated with anti-endothelial cells antibodies (AECA), there is a widespread awareness of the limitations of the tests currently in use. Our objective was therefore to establish clones, in the hope that some of them would express disease-specific membrane autoantigens. Two EC lines and 7 clones were estab lished by fusing human umbilical vein EC with epithelial A549/8 cells, and cloning by limit ing dilution. An additional clone was derived from the EA.hy 926 cell line. All clones carried EC markers, such as thrombomoduline (TM) and platelet-EC adhesion molecule 1 but differed from each other, depending on whether they expressed HLA class II antigen, LFA-1, thrombospondin receptor or von Willebrand factor (vWf) antigen. Clones were also characterized by their ability to release tissue plasminogen activator, interleukin 6, TM and vWf. This panel is meant to distinguish reactivities of AECA.

Key Words: endothelial cell • anti-endothelial cells antibody

Lupus, Vol. 5, No. 2, 103-112 (1996)
DOI: 10.1177/096120339600500204
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Ann Rheum DisHome page
C Mihai and J W C Tervaert
Anti-endothelial cell antibodies in systemic sclerosis
Ann Rheum Dis, February 1, 2010; 69(2): 319 - 324.
[Abstract] [Full Text] [PDF]


Advertisement