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In vitro type-1 and type-2 cytokine production in systemic lupus erythematosus: lack of relationship with clinical disease activity

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

GP Rizzardi

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

MO Borghi

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

F. Nicoletti

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

C. Fain

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

N. Del Papa

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

PL Meroni

Institute of Internal Medicine, Immunopathology and Infectious Diseases, University of Milan, Italy

Objective: to investigate the relationship between disease activity and in vitro cytokine, soluble(s)CD23 and polyclonal and anti-DNA antibody production by PBMC from patients with active systemic lupus erythematosus (SLE).

Methods: cytokines, sCD23 and immunoglobulins were estimated by ELISA in unstimulated and polyclonal mitogen-stimulated culture supernatants.

Results: PHA-induced IL-2 and IFN-y production were decreased, whereas spontaneous and PHA-induced IL-6 and IL-10 production were increased in cultures of SLE lympho cytes. Conversely, spontaneous and PHA-stimulated IL-4 and sCD23 production was com parable between patients and controls. Finally, we found an increase in in vitro spontaneous polyclonal and anti-DNA IgG secretion.

Conclusions: We demonstrated an expanded type-2 cytokine profile with no correlation with parameters of disease activity.

Key Words: SLE • sCD23 • cytokines • polyclonal B cell activation

Lupus, Vol. 5, No. 2, 139-145 (1996)
DOI: 10.1177/096120339600500209


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