This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waisman, A. Articles by Mozes, E. Search for Related Content PubMed Articles by Waisman, A. Articles by Mozes, E. Social Bookmarking                   What's this?

Review : Variable regions of two murine antibodies that bind the SLE associated 16/6 idiotype

Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel

P.J. Ruiz

Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel

E. Mozes

Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel

Experimental systemic lupus erythematosus can be induced in naive mice of different strains using a human monoclonal antibody bearing the 16/6 idiotype and a murine anti-16/6 Id monoclonal antibody designated 1A3-2. Herein we report the isolation of a second anti-16/6 Id antibody, 3F7-8, from BALB/c mice afflicted with experimental SLE. In contrast to the previously reported (1A3-2) anti-16/6 Id monoclonal antibody, mAb 3F7-8 does not induce experimental SLE upon immunization. The variable heavy and light chains of both anti bodies were cloned and their sequences were determined. The V_H of mAb 1A3-2 was found to express a germ line gene from the Q52 family, with a high homology to an anti-lysozyme antibody. The VH of monoclonal antibody 3F7-8 was found to express a 7183 germ line gene, showing over 95% homology with the V_H of 12 anti-Sm antibodies isolated from MRL-1pr mice. Based on sequence homology to other known antibodies, we further demon strated that monoclonal antibodies 1A3-2 and 3F7-8 bind lysozyme and the Sm ribonu cleoproteins, respectively, in addition to their binding to the 16/6 Id.

Key Words: mAb sequence • 16/6 idiotype • SLE • idiotypic network

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Blank, A. Waisman, E. Mozes, T. Koike, and Y. Shoenfeld Characteristics and pathogenic role of anti-{beta}2-glycoprotein I single-chain Fv domains: induction of experimental antiphospholipid syndrome Int. Immunol., December 1, 1999; 11(12): 1917 - 1926. [Abstract] [Full Text] [PDF]