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Detection of restricted junctional diversity of peripheral T cells in SLE patients by spectratyping

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

M. Herrmann

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

BB Ponner

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

R. Voll

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

P. Kern

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

C. Frank

Department of Paediatrics, Friedrich-Alexander University of Erlangen, 91054 Erlangen, Germany

JR Kalden

Institute of Clinical Immunology, Department of Internal Medicine , Friedrich-Alexander University of Erlangen-Nuremberg

Analysis of somatic mutations revealed that anti-double-stranded DNA (dsDNA) autoantibodies from patients with systemic lupus erythematosus (SLE) share features of a T cell dependent, antigen driven immune response. Therefore we analysed the length diversity of the complementarity determining region 3 (CDR3) of T cell receptor (TCR) by high resolution gel electrophoresis of 16 Vß family specific RT PCR products (spectratyping). To enable statistical analysis we developed a quantitative scoring method for the histograms. We investigated 16 Vß gene families in peripheral T cells of SLE patients (n = 9) with active (n = 5) and inactive (n = 4) disease as well as normal healthy blood donors (NHD; n = 9). Analysis of TCR Vß spectratypes (active SLE, n = 59; inactive SLE, n = 51 and NHD n = 97) revealed statistically significant differences of CDR3 length distribution between SLE patients and NHD (P < 0.0001 (active SLE/NHD) and P = 0.0034 (inactive SLE/NHD). These results suggest that spectratyping is able to detect clonal activation of peripheral T cells which correlates to disease activity in SLE patients. We conclude that peripheral T cells from SLE patients display features of a secondary antigen driven immune response.

Key Words: Spectratyping • TCR ß-chain • oligoclonal T cell expansion

Lupus, Vol. 6, No. 9, 701-707 (1997)
DOI: 10.1177/096120339700600904


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