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Two populations of endothelial cell antibodies cross-react with heparin

R Revelen

A Bordron

Laboratory of Immunology, Brest University Medical School, Brest, France

D Mottier

Department of Internal Medicine, Brest University Medical School, Brest, France

P Youinou

R Le Corre

Laboratory of Immunology, Brest University Medical School, Brest, France

As endothelial cells (EC) express heparin-like glycosaminoglycans, such as heparan sulfate, it was essential to investigate the relation of anti-EC antibody (AECA) to heparin reactivity. AECA were detected in 43 of 131 autoimmune sera and anti-heparin antibodies (AHA) in 25. These autoimmune reactivities were significantly associated (P corrected < 0.0005). Seven AECA-positive/AHA-positive and three AECA-negative/AHA-positive sera were affinity-purified using protein G column followed by a heparin-Sepharose column. Two populations of AECA were recovered from the second column. One was eluted with 0.4 M NaCl which bound to EC and to solid-phase heparin with low affinity, but not to soluble heparin. The second population of AECA, which was eluted with 4 M guanidine HCl=2 M NaCl, recognized EC and solid-phase heparin with high affinity, but also soluble heparin. The latter population of AECA might thus be an important cause of autoimmune vascular thrombosis in systemic diseases.

Key Words: endothelial cells • antibodies • heparin

Lupus, Vol. 7, No. 2, 86-94 (1998)
DOI: 10.1191/096120398678919769


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