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Thrombogenic properties of antiphospholipid antibodies do not depend on their binding to ß2 glycoprotein 1 (ß2GP1) alone

S S Pierangeli

E E Gharavi

Departments of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, GA

T Hua

Department of Medicine, Louisiana State University, New Orleans, LA

X W Liu

Departments of Physiology, Morehouse School of Medicine, Atlanta, GA

J H Barker

Departments of Surgery, University of Louisville, Louisville, KY, USA

G H Anderson

Departments of Physiology, University of Louisville, Louisville, KY, USA

E N Harris

Departments of Internal Medicine, Morehouse School of Medicine, Atlanta, GA

Immunization of mice with ß₂glycoprotein 1 (ß₂GP1) induces production of antiphospholipid (aPL) antibodies, which were shown to have thrombus enhancing properties in an experimental mouse model, indicating that these antibodies are thrombogenic in vivo. To determine whether the thrombogenic effect of murine antiphospholipid antibodies is due to their aPL or their anti-ß₂GP1 activity, we injected mice with murine monoclonal anticardiolipin (aCL) and anti-ß₂GP1 antibodies. Effects of these antibodies on thrombus formation, was evaluated utilizing a mouse model which enables kinetics of thrombus formation to be studied. The results of this study showed that the size of the thrombus in animals injected with murine aCL antibodies was larger than that in control groups. There was no difference in thrombus kinetics between anti-ß₂GP1 injected mice and controls, suggesting that the thrombogenic effect of aPL antibodies is not related to their anti-b2GP1 activity alone. Mice receiving monoclonal antibodies with both aCL and anti-ß₂GP1 activity, also increase thrombus size when compared with controls. These data indicate that murine aCL, but not anti-ß₂GP1, antibodies are thrombogenic in vivo.

Key Words: anticardiolipin antibodies • anti-ß2GPI antibodies • antiphospholipid syndrome • murine monoclonal antibodies • lupus anticoagulant

Lupus, Vol. 7, No. 5, 341-346 (1998)
DOI: 10.1191/096120398678920190


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