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Induction of antiphospholipid antibodies by immunization with synthetic viral and bacterial peptides

Morehouse School of Medicine, Atlanta, GA, USA

H Chaimovich

Chemistry Institute, University of Sao Paulo, Brazil

E Cucurull

Department of Medicine, Section of Rheumatology, Louisiana State University Medical Center, New Orleans, LA, USA

C M Celli

Chemistry Institute, University of Sao Paulo, Brazil

H Tang

W A Wilson

Department of Medicine, Section of Rheumatology, Louisiana State University Medical Center, New Orleans, LA, USA

A E Gharavi

Morehouse School of Medicine, Atlanta, GA, USA

We previously induced pathogenic antibodies against anionic phospholipids (PL) in experimental animals by immunization with lipid-free purified human ß₂glycoprotein I (ß₂GPI). We hypothesized that antiphospholipid antibodies (aPL) are induced by in vivo binding of foreign b2GPI to self-PL, thus forming an immunogenic complex against which aPL antibodies are produced. If this hypothesis is true, other PL-binding proteins that are products of ubiquitous viral/bacterial agents may also induce aPL. To test this hypothesis, groups of NIH/Swiss mice were immunized with synthetic peptides of viral and bacterial origin that share structural similarity with the putative PL-binding region of ß₂GPI. Compared with the control groups, animals immunized with the peptides produced significantly higher levels of aPL and anti-ß₂GPI antibodies. These findings demonstrate that some PL-binding viral and bacterial proteins function like ß₂GPI in inducing aPL and anti-ß₂GPI production, and are consistent with a role for such viral and bacterial proteins in inducing aPL antibody production in humans.

Key Words: antiphospholipid antibodies • ß2glycoprotein I • viral and bacterial peptides • antiphospholipid syndrome • systemic lupus erythematosus

Lupus, Vol. 8, No. 6, 449-455 (1999)
DOI: 10.1177/096120339900800607


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