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HLA class II alleles associations of anticardiolipin and anti b2GPI antibodies in a large series of European patients with systemic lupus erythematosus

Istituto di Reumatologia, UniversitaÁ di Siena, Italy; Institute of Rheumatology—Policlinico "Le Scotte" —Viale Bracci, 53100 Siena, Italy

Gian Domenico Sebastiani

Divisione di Reumatologia, Ospedale San Camillo, Roma, Italy

Angela Tincani

Servizio di Immunologia Clinica, Spedali Civili, Brescia, Italy

Jean-Charles Piette

Service Medecine Interne, CHU Pitié-SalpetrieÁre, Paris, France

Flavio Allegri

Servizio di Immunologia Clinica, Spedali Civili, Brescia, Italy

Gabriella Morozzi

Francesca Bellisai

Istituto di Reumatologia, UniversitaÁ di Siena, Italy

Raffaella Scorza

Laboratorio di Immunologia Cellulare e Immunogenetica, Istituto di Medicina Interna, Malattie Infettive e Immunopatologia, Universita Á di Milano, Italy

Giovanni Battista Ferrara

Laboratorio di Immunogenetica, Centro di Biotecnole gie Avanz TE, Genova, Italy

Carlo Carcassi

Dipartimento di Genetica Medica, Istituto di Clinica Medica, UniversitaÁ di Cagliari, Italy

Josep Font

Unitat de Malalties Autoimmunes, Sistemiques, Hospital Clinic, Barcelona, Spain

Giuseppe Passiu

II Cattedra di Reumatologia, Dipartimento di Scienze Mediche, Centro Malattie Reumatiche Sistemiche, Á di Cagliari, Italy

Josef Smolen

2nd Department of Medicine, Center for Rheumatic Diseases, Lainz Hospital, Vienna, Austria

Chryssa Papasteriades

Department of Immunology and Histocompatibility, Evangelismos Hospital, Athens, Greece

Frederic Houssiau

Service du Rhumatologie, Cliniques Universitaires Saint-Luc Université Catholique de Louvain, Bruxelles, Belgium

Antonio Fernandez Nebro

Departement of Medicine, Rheumatology Service, Section Lupus Unit, Universitary Hospital, University of Malaga, Spain

Enrique De Ramon Garrido

Unidad de Enfermedades Autoimmunes Sistemicas, Hospital Regional del SAS, Servicio de Medicina Interna, Malaga, Spain

Anna Jedryka-Goral

Department of Connective Tissue Diseases, Institute of Rheumatology, Warsaw, Poland

Roberto Marcolongo

Istituto di Reumatologia, UniversitaÁ di Siena, Italy

European Concerted Action on the Immunogenetics of SLE

The objective of this study was to determine the HLA class II associations of the anticardiolipin (aCL) and anti-b2GPI (ab2GPI) antibodies in a large series of European patients with systemic lupus erythematosus (SLE).

A cohort of 577 European SLE patients was enrolled. aCL and ab2GPI were measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB5, DQA1 and DQB1 loci was performed by the polymerase chain reaction–sequence specific oligonucleotide probes (PCR–SSOP) method.

aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of patients, respectively. IgG and IgM ab2GPI were detected in 20% of patients. aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*0403, DRB1*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative association with DQA1*0501, DRB3*0202. ab2GPI showed positive association with DRB1*0402, DRB1*0403, DQB1*0302. DRB1*0402 carried the highest relative risk for the presence of both aCL (RR = 8.1) and ab2GPI (RR = 4.6).

Our results confirm the already described associations of aCL with HLA DR4 and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus, the *0402 was most represented both in aCL and in ab2GPI positive patients. In addition, HLA class II associations of ab2GPI are for the first time extensively examined in a large cohort of European SLE patients.

Key Words: SLE • HLA class II • anticardiolipin antibodies • antib2GPI antibodies

Lupus, Vol. 9, No. 1, 47-55 (2000)
DOI: 10.1177/096120330000900109


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