This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Satake, F Articles by Kubota, T Search for Related Content PubMed PubMed Citation Articles by Satake, F Articles by Kubota, T Social Bookmarking                   What's this?

Induction of anti-DNA antibodies by immunization with anti-DNA antibodies: mechanism and characterization

School of Allied Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan; Gunma University School of Health Science, Maebashi, Japan.

N Watanabe

School of Allied Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan

N Miyasaka

The First Department of Medicine, Tokyo Medical and Dental University, Tokyo, Japan

Y Kanai

Institute of Medical Sciences, The University of Tokyo, Tokyo, Japan

T Kubota

Tokyo Medical and Dental University School of Allied Health Sciences, 1-5-45 Yushima, Bunkyo, Tokyo 113-8519, Japan. Tel/Fax: (/ 81) 3 5803 5369; tetsuo.kubota.mtec{at}med.tmd.ac.jp

Two well-characterized IgG monoclonal antibodies, reactive with double-stranded (ds) DNA and nucleosomes, were administered to normal BALB/c mice to examine the reproducibility and the biology of a previously reported model of anti-DNA antibody induction by immunization with antiDNA antibodies. The monoclonal antibodies were purified either with or without a high-salt wash to remove nucleosomal antigens bound to them during the cell culture. Both monoclonal antibodies, but not normal IgG, induced significant IgG anti-dsDNA antibody production from 1 week to 25 weeks after the last immunization. The antibodies produced in this manner possess different binding preferences to ds synthetic polynucleotides than the antibodies used for the immunization, and they did not react with nucleosomes. The monoclonal antibodies purified with the high-salt wash were more effective in anti-DNA antibody induction than those purified without the high-salt wash. Even when bound to these monoclonal antibodies, neither dsDNA, nucleosomes, or ds synthetic polynucleotides exert significant antigenicity. For example, anti-DNA antibodies produced by mice immunized with an immune complex formed by poly(dA-dT) and one of the monoclonal antibodies that has a high affinity to this polynucleotide did not show an increased affinity to poly(dA-dT). Together, these results suggest that anti-DNA antibody molecules or processed antibody peptides, and not DNA/nucleosomes carried by anti-DNA antibodies, play a role in this model of anti-DNA antibody production.

Key Words: purification of anti-DNA antibodies • induction of anti-DNA antibodies • systemic lupus erythematosus • idiotypic network

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Blank and Y. Shoenfeld Experimental models of systemic lupus erythematosus: anti-dsDNA in murine lupus Rheumatology, September 1, 2005; 44(9): 1086 - 1089. [Full Text] [PDF]

				D Isenberg, D Katz, P Maddison, R Watts, L Tucker, and A Cooke Induction of anti-DNA antibodies: commentary on article by Satake et al Lupus, January 1, 2001; 10(1): 63 - 65. [PDF]

				Y Shoenfeld Eppur si muove (Galileo Galilei 1564-1642): the idiotypic dysregulation of autoantibodies as part of the etiology of SLE Lupus, September 1, 2000; 9(7): 481 - 483. [PDF]