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Antibodies to human myeloperoxidase in glomerular immune deposits of systemic lupus erythematosus

Division of Rheumatology, Department of Medicine, University of Washington, Seattle, WA, USA; Division of Rheumatology, Department of Medicine, Box 356428, University of Washington, Seattle, WA 98195-6428, USA. Tel: (+1) 206 534 3414, Fax: (+1) 206 685 9397; mannik{at}u.washington.edu

C E Merrill

Division of Rheumatology, Department of Medicine, University of Washington, Seattle, WA, USA

M H Wener

Department of Laboratory Medicine, University of Washington, Seattle, WA, USA

Antibodies to human myeloperoxidase and cathepsin G have been detected in the serum of some patients with systemic lupus erythematosus. Therefore, the presence of antibodies to human myeloperoxidase and cathepsin G was examined in glomerular immune deposits. Glomerular basement membrane fragments were prepared from renal tissues obtained at autopsy from 19 patients with systemic lupus erythematosus. IgG was extracted from the glomerular basement membrane fragments and tested with sensitive immunoassays for antibodies to myeloperoxidase and cathepsin G. Antibodies to cathepsin G were not detected in the extracts but antibodies to human myeloperoxidase were found in extracts of one specimen. In the extract with 6M guanidine hydrochloride these antibodies were enriched 103-fold, compared to the initial supernatant of glomeruli, which served as a serum surrogate. The recovered antibodies to myeloperoxidase accounted for 12% of the recovered IgG. These findings add autoantibodies to human myeloperoxidase to the list of antibodies that have been shown to be present in glomerular immune deposits of patients with lupus glomerulonephritis.

Key Words: lupus nephritis • antibodies to myeloperoxidase • systemic lupus erythematosus

Lupus, Vol. 9, No. 8, 607-613 (2000)
DOI: 10.1191/096120300678828758


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