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Telomere length analysis in monocytes and lymphocytes from patients with systemic lupus erythematosus using multi-color flow-FISH
F. Beier
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany
S. Balabanov
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Hematology and Oncology, University Hospital Eppendorf, Hamburg, Germany
C.C. Amberger
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany
U. Hartmann
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Pharmacology, University of Tubingen, Germany
K. Manger
Department of Rheumatology, University of Erlangen, Germany
K. Dietz
Department of Medical Biometry, University of Tubingen, Germany
I. Kötter
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany
T.H. Brummendorf
Division of Hematology, Oncology and Immunology, University of Tubingen, Germany, Department of Hematology and Oncology, University Hospital Eppendorf, Hamburg, Germany, t.bruemmendorf{at}uke.uni-hamburg.de
In order to analyse telomere length in subsets of human peripheral blood lymphocytes and monocytes, we modified a recently developed multicolor flow- fluorescent in situ hybridization (FISH) methodology that combines flow-FISH and antibody staining for cell surface antigens. We analysed telomere length of peripheral blood mononuclear cells in a group of 22 patients with systemic lupus erythematosus (SLE) and 20 age-matched healthy donors. We found that neither CD4+, CD8+, CD19+ cells nor CD14+ monocytes have significantly shorter telomeres compared with their healthy counterparts. On the basis of these findings, we then used monocyte telomere length as internal reference in order to control for intra-individual variability in telomere length. By using this approach, we could demonstrate significant telomere shortening in all three lymphocyte subsets (in all cases P < 0.05) compared with monocytes. However, these differences did not vary significantly between SLE patients and controls. In summary, telomere lengths in subpopulations of hematopoietic cells can be monitored in patients with SLE using multicolor flow-FISH. While confirming data by other groups on telomere length in lymphocyte subpopulations, our data argue against an increased proliferation rate of peripheral blood monocytes reflected by accelerated telomere shortening in patients with SLE. Lupus (2007) 16, 955—962.
Key Words: flow-FISH • monocyte • systemic lupus erythematosus • telomere • telomerase
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Lupus, Vol. 16, No. 12,
955-962 (2007)
DOI: 10.1177/0961203307084299
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